Review



emsa/gel-shift binding buffer gs005  (Beyotime)


Bioz Verified Symbol Beyotime is a verified supplier
Bioz Manufacturer Symbol Beyotime manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 90

    Structured Review

    Beyotime emsa/gel-shift binding buffer gs005
    Emsa/Gel Shift Binding Buffer Gs005, supplied by Beyotime, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/gel-shift+binding+buffer/chemiluminescent+emsa+kit+gs009/pm40514371-304-12-16
    Average 90 stars, based on 1 article reviews
    emsa/gel-shift binding buffer gs005 - by Bioz Stars, 2026-09
    90/100 stars

    Images

    Related Articles

    Incubation:

    Article Title: IGF-1-induced enhancement of PRNP expression depends on the negative regulation of transcription factor FOXO3a.
    Article Snippet: 1 mM biotin-labeled probes were incubated with 10 mg,15 mg or 20 mg HeLa cell nuclear extracts (NEs) in gel-shift binding buffer (poly[dI-dC], DTT, glycerol, EDTA, NaCl, MgCl2, Tris, Beyotime, GS009-1) at room temperature for 20 minutes and then loaded onto a 6% polyacrylamide gel in 0.56TBE buffer for electrophoresis on ice at 120 V for approximately 1 hour.

    Article Title: Identification of a novel forkhead transcription factor MtFKH1 for cellulase and xylanase gene expression in Myceliophthora thermophila (ATCC 42464)
    Article Snippet: In each EMSA reaction, constant amount (100 ng) of the DNA probes were incubated with gradual quantities (0-0.2 μg) of recombinant proteins individually at 25°C for 30 min in solution containing Gel-Shift Binding Buffer (Beyotime Biotech, Shanghai, China).

    Article Title: Investigating the impact of local inflammation on granulosa cells and follicular development in women with ovarian endometriosis.
    Article Snippet: The reaction mixture for each sample contained 2 mL of gel-shift binding buffer (5 ), 5 mg of the nuclear fraction, and 0.5 mL of NF-kB consensus oligo (50-AGT TGA GGG GAC TTT CCC AGG C-30, 30-TCA ACT CCC CTG AAA GGG TCC G-50) biotin-labeled probes (Beyotime), adjusted to 10 mL by nuclease-free water; the mixture then was incubated at room temperature for 30 minutes before we added 1 mL of loading buffer (10 ).

    Recombinant:

    Article Title: IGF-1-induced enhancement of PRNP expression depends on the negative regulation of transcription factor FOXO3a.
    Article Snippet: 1 mM biotin-labeled probes were incubated with 10 mg,15 mg or 20 mg HeLa cell nuclear extracts (NEs) in gel-shift binding buffer (poly[dI-dC], DTT, glycerol, EDTA, NaCl, MgCl2, Tris, Beyotime, GS009-1) at room temperature for 20 minutes and then loaded onto a 6% polyacrylamide gel in 0.56TBE buffer for electrophoresis on ice at 120 V for approximately 1 hour.

    Article Title: Identification of a novel forkhead transcription factor MtFKH1 for cellulase and xylanase gene expression in Myceliophthora thermophila (ATCC 42464)
    Article Snippet: In each EMSA reaction, constant amount (100 ng) of the DNA probes were incubated with gradual quantities (0-0.2 μg) of recombinant proteins individually at 25°C for 30 min in solution containing Gel-Shift Binding Buffer (Beyotime Biotech, Shanghai, China).

    Article Title: Investigating the impact of local inflammation on granulosa cells and follicular development in women with ovarian endometriosis.
    Article Snippet: The reaction mixture for each sample contained 2 mL of gel-shift binding buffer (5 ), 5 mg of the nuclear fraction, and 0.5 mL of NF-kB consensus oligo (50-AGT TGA GGG GAC TTT CCC AGG C-30, 30-TCA ACT CCC CTG AAA GGG TCC G-50) biotin-labeled probes (Beyotime), adjusted to 10 mL by nuclease-free water; the mixture then was incubated at room temperature for 30 minutes before we added 1 mL of loading buffer (10 ).

    Gel Shift:

    Article Title: IGF-1-induced enhancement of PRNP expression depends on the negative regulation of transcription factor FOXO3a.
    Article Snippet: 1 mM biotin-labeled probes were incubated with 10 mg,15 mg or 20 mg HeLa cell nuclear extracts (NEs) in gel-shift binding buffer (poly[dI-dC], DTT, glycerol, EDTA, NaCl, MgCl2, Tris, Beyotime, GS009-1) at room temperature for 20 minutes and then loaded onto a 6% polyacrylamide gel in 0.56TBE buffer for electrophoresis on ice at 120 V for approximately 1 hour.

    Article Title: Identification of a novel forkhead transcription factor MtFKH1 for cellulase and xylanase gene expression in Myceliophthora thermophila (ATCC 42464)
    Article Snippet: In each EMSA reaction, constant amount (100 ng) of the DNA probes were incubated with gradual quantities (0-0.2 μg) of recombinant proteins individually at 25°C for 30 min in solution containing Gel-Shift Binding Buffer (Beyotime Biotech, Shanghai, China).

    Article Title: Investigating the impact of local inflammation on granulosa cells and follicular development in women with ovarian endometriosis.
    Article Snippet: The reaction mixture for each sample contained 2 mL of gel-shift binding buffer (5 ), 5 mg of the nuclear fraction, and 0.5 mL of NF-kB consensus oligo (50-AGT TGA GGG GAC TTT CCC AGG C-30, 30-TCA ACT CCC CTG AAA GGG TCC G-50) biotin-labeled probes (Beyotime), adjusted to 10 mL by nuclease-free water; the mixture then was incubated at room temperature for 30 minutes before we added 1 mL of loading buffer (10 ).

    Binding Assay:

    Article Title: IGF-1-induced enhancement of PRNP expression depends on the negative regulation of transcription factor FOXO3a.
    Article Snippet: 1 mM biotin-labeled probes were incubated with 10 mg,15 mg or 20 mg HeLa cell nuclear extracts (NEs) in gel-shift binding buffer (poly[dI-dC], DTT, glycerol, EDTA, NaCl, MgCl2, Tris, Beyotime, GS009-1) at room temperature for 20 minutes and then loaded onto a 6% polyacrylamide gel in 0.56TBE buffer for electrophoresis on ice at 120 V for approximately 1 hour.

    Article Title: Identification of a novel forkhead transcription factor MtFKH1 for cellulase and xylanase gene expression in Myceliophthora thermophila (ATCC 42464)
    Article Snippet: In each EMSA reaction, constant amount (100 ng) of the DNA probes were incubated with gradual quantities (0-0.2 μg) of recombinant proteins individually at 25°C for 30 min in solution containing Gel-Shift Binding Buffer (Beyotime Biotech, Shanghai, China).

    Article Title: Investigating the impact of local inflammation on granulosa cells and follicular development in women with ovarian endometriosis.
    Article Snippet: The reaction mixture for each sample contained 2 mL of gel-shift binding buffer (5 ), 5 mg of the nuclear fraction, and 0.5 mL of NF-kB consensus oligo (50-AGT TGA GGG GAC TTT CCC AGG C-30, 30-TCA ACT CCC CTG AAA GGG TCC G-50) biotin-labeled probes (Beyotime), adjusted to 10 mL by nuclease-free water; the mixture then was incubated at room temperature for 30 minutes before we added 1 mL of loading buffer (10 ).

    Countercurrent Chromatography:

    Article Title: IGF-1-induced enhancement of PRNP expression depends on the negative regulation of transcription factor FOXO3a.
    Article Snippet: 1 mM biotin-labeled probes were incubated with 10 mg,15 mg or 20 mg HeLa cell nuclear extracts (NEs) in gel-shift binding buffer (poly[dI-dC], DTT, glycerol, EDTA, NaCl, MgCl2, Tris, Beyotime, GS009-1) at room temperature for 20 minutes and then loaded onto a 6% polyacrylamide gel in 0.56TBE buffer for electrophoresis on ice at 120 V for approximately 1 hour.

    Article Title: Identification of a novel forkhead transcription factor MtFKH1 for cellulase and xylanase gene expression in Myceliophthora thermophila (ATCC 42464)
    Article Snippet: In each EMSA reaction, constant amount (100 ng) of the DNA probes were incubated with gradual quantities (0-0.2 μg) of recombinant proteins individually at 25°C for 30 min in solution containing Gel-Shift Binding Buffer (Beyotime Biotech, Shanghai, China).

    Article Title: Investigating the impact of local inflammation on granulosa cells and follicular development in women with ovarian endometriosis.
    Article Snippet: The reaction mixture for each sample contained 2 mL of gel-shift binding buffer (5 ), 5 mg of the nuclear fraction, and 0.5 mL of NF-kB consensus oligo (50-AGT TGA GGG GAC TTT CCC AGG C-30, 30-TCA ACT CCC CTG AAA GGG TCC G-50) biotin-labeled probes (Beyotime), adjusted to 10 mL by nuclease-free water; the mixture then was incubated at room temperature for 30 minutes before we added 1 mL of loading buffer (10 ).

    Electrophoresis:

    Article Title: IGF-1-induced enhancement of PRNP expression depends on the negative regulation of transcription factor FOXO3a.
    Article Snippet: 1 mM biotin-labeled probes were incubated with 10 mg,15 mg or 20 mg HeLa cell nuclear extracts (NEs) in gel-shift binding buffer (poly[dI-dC], DTT, glycerol, EDTA, NaCl, MgCl2, Tris, Beyotime, GS009-1) at room temperature for 20 minutes and then loaded onto a 6% polyacrylamide gel in 0.56TBE buffer for electrophoresis on ice at 120 V for approximately 1 hour.

    Article Title: Identification of a novel forkhead transcription factor MtFKH1 for cellulase and xylanase gene expression in Myceliophthora thermophila (ATCC 42464)
    Article Snippet: In each EMSA reaction, constant amount (100 ng) of the DNA probes were incubated with gradual quantities (0-0.2 μg) of recombinant proteins individually at 25°C for 30 min in solution containing Gel-Shift Binding Buffer (Beyotime Biotech, Shanghai, China).

    Article Title: Investigating the impact of local inflammation on granulosa cells and follicular development in women with ovarian endometriosis.
    Article Snippet: The reaction mixture for each sample contained 2 mL of gel-shift binding buffer (5 ), 5 mg of the nuclear fraction, and 0.5 mL of NF-kB consensus oligo (50-AGT TGA GGG GAC TTT CCC AGG C-30, 30-TCA ACT CCC CTG AAA GGG TCC G-50) biotin-labeled probes (Beyotime), adjusted to 10 mL by nuclease-free water; the mixture then was incubated at room temperature for 30 minutes before we added 1 mL of loading buffer (10 ).



    Similar Products

    90
    Beyotime emsa/gel-shift binding buffer gs005
    Emsa/Gel Shift Binding Buffer Gs005, supplied by Beyotime, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/gel-shift+binding+buffer/chemiluminescent+emsa+kit+gs009/pm40514371-304-12-16
    Average 90 stars, based on 1 article reviews
    emsa/gel-shift binding buffer gs005 - by Bioz Stars, 2026-09
    90/100 stars
      Buy from Supplier

    90
    Beyotime gel-shift binding buffer
    Gel Shift Binding Buffer, supplied by Beyotime, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/gel-shift+binding+buffer/chemiluminescent+emsa+kit+gs009/10__1016_slash_j__micres__2025__128097-141-31-34
    Average 90 stars, based on 1 article reviews
    gel-shift binding buffer - by Bioz Stars, 2026-09
    90/100 stars
      Buy from Supplier

    90
    Beyotime emsa/gel-shift binding buffer gs009
    Emsa/Gel Shift Binding Buffer Gs009, supplied by Beyotime, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/gel-shift+binding+buffer/chemiluminescent+emsa+kit+gs009/pm39775883-97-23-27
    Average 90 stars, based on 1 article reviews
    emsa/gel-shift binding buffer gs009 - by Bioz Stars, 2026-09
    90/100 stars
      Buy from Supplier

    90
    Beyotime emsa/gel-shift binding buffer
    Emsa/Gel Shift Binding Buffer, supplied by Beyotime, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/gel-shift+binding+buffer/chemiluminescent+emsa+kit+gs009/pm40261004-396-13-16
    Average 90 stars, based on 1 article reviews
    emsa/gel-shift binding buffer - by Bioz Stars, 2026-09
    90/100 stars
      Buy from Supplier

    90
    Beyotime 5×emsa/gel-shift binding buffer
    5×Emsa/Gel Shift Binding Buffer, supplied by Beyotime, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/gel-shift+binding+buffer/chemiluminescent+emsa+kit+gs009/pm40195781-114-25-28
    Average 90 stars, based on 1 article reviews
    5×emsa/gel-shift binding buffer - by Bioz Stars, 2026-09
    90/100 stars
      Buy from Supplier

    90
    Beyotime ems a / gel-shift binding buffer
    Ems A / Gel Shift Binding Buffer, supplied by Beyotime, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/gel-shift+binding+buffer/chemiluminescent+emsa+kit+gs009/pm40167330-141-18-24
    Average 90 stars, based on 1 article reviews
    ems a / gel-shift binding buffer - by Bioz Stars, 2026-09
    90/100 stars
      Buy from Supplier

    Image Search Results